Plots a histogram of the no. of pixels with a given value within the selected area vs. the pixel value. Quite useful when trying to optimize the contrast or brightness of an image.
Clicking capture converts the graph into a new image.
Clicking subtract baseline allows you to subtract a baseline from the histogram. Select the desired baseline by clicking as many times as desired to create the baseline curve. The baseline curve is constructed using a B-spline (see under B-spline curve ). You can modify the curve as desired by clicking and dragging the small boxes (which represent control points for the curve). Up to 200 control points can be used. The control points do not have to be within the graph region; however, any negative numbers created by baseline subtraction are truncated to 0's.
See strip densitometry above for more details on the plot functions.
Note: When a graph is being displayed, smoothing, subtracting baseline, etc., affect the displayed data only, and have no effect on the image.