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Peak Areas

Finds the x and y coordinates of each peak and calculates the peak area. The integration limits are shown as short black lines on either side of each peak. No baseline subtraction is carried out before calculating peak areas. Thus, best results are usually obtained if a baseline was first subtracted by clicking on `Auto.baseline' or `Manual baseline'.

When the list of peaks is displayed, most of the click buttons on the left side of the plot are still active, but now apply to the peak list. This includes:

Save to disk

Capture image

Help

OK

The other buttons (`peak areas',`smooth', `manual baseline', and `auto baseline') are inactivated while displaying the list.

In the DOS version, the peak peak area list can also be closed by clicking on the `-' symbol in the upper left corner.

Area calculations: While the graph is displayed, it is also possible to manually measure peak areas by selecting the desired region of the graph. To select a region, click with the left mouse button and drag horizontally within the graph area. When the mouse button is released, the total area of the selected region is automatically displayed.


next up previous contents index
Next: Western Blots and Stained Up: Strip Densitometry Previous: Curve Densitometry   Contents   Index
root 2008-10-10